VLS PCR-X Respiratory Disease Panel

Pathogen Identification for Respiratory Disease Panel

Beef & Dairy Cattle, Sheep & Goats
x18 PATHOGENS
Nasal swab SAMPLE TYPE
48 hrs* TURNAROUND

Full Disease & Pathogen Target List • 18 Pathogens

BACTERIA • 8

  • Pasteurella multocida
  • Histophilus somni
  • Mannheimia haemolytica
  • Trueperella pyogenes
  • Mycobacterium tuberculosis MTBC Exotic
  • Bibersteinia trehalosi
  • Mycoplasma spp.
  • Ureaplasma diversum

VIRUS • 10

  • Peste des petits ruminants virus Exotic
  • Bovine coronavirus
  • Bovine respiratory syncytial virus
  • Bovine viral diarrhoea virus
  • Bovine parainfluenza (PI-3)
  • Bovine alphaherpesvirus
  • Maedi-Visna Virus Exotic
  • Bovine rhinitis virus A-B
  • Bovine adenovirus
  • Bovine influenza D virus

Exotic = exotic disease, not currently established / present in Australia.

VLS PCR-X = qPCR qualitative results

Sample Collection Guide

  • Primary sample

    Nasal swab

  • What to use

    Flocked (nylon, polyester, rayon tip), plastic shaft dry swab. Alternative swabs such as plastic shaft cotton may be used.

  • How to store

    Cold (fridge): 24-48 hrs max. Advise Freezing (-20°C) if available, for >48 hr delays.**

    **Transportation / storage: Timing matters for PCR test sensitivity. Refrigerate immediately after collection. Transport within 24 hours (faeces) & 24-48 hours (Swabs / Milk) if possible. If that’s not possible, VLS advises freezing (-20°C) where possible and to avoid thawing.

  • How to collect

    Gently put swab into nostril, turn it a few times, seal in tube.***

    ***Swabs per site: collect 1 swab minimum per site. 2-3 swabs preferred to enable re-confirmation if needed.

*From laboratory receipt.

Golden Rules

  • Nylon (flocked) and plastic shaft cotton swabs will ensure quality results.
  • Even a dry swab must go into the tube given — do not send it loose or wrapped in paper.
  • Close tubes and containers tightly. Only open to add the sample.
  • Keep every sample cold (fridge) right after collection. Avoid hot environments.
  • Write the animal’s name / number and today’s date on every sample.
  • If transport is delayed, freeze— avoid >48 hour at fridge temperature.